CHOLESTEROL CRYSTAL
Crystals are the precipitated three dimensional solid structure obtained from saturated solution. The characteristics of a compound may be determined by the shape of the crystal. Therefore, a crystalline structure is a marker to identify a chemical substance.
REAGENTS & GLASSWARES:
1. Alcohol
2. Cholesterol
3. Microscope
4. Test tubes, test tube stands, test tube holder, distilled water, pipette with sucker, beakers and graduated cylinder.
PROCEDURE:
1. Take a neat test tube and add 2 mL of pure alcohol.
2. Add a pinch of cholesterol, mix and heat it gently with addition of cholesterol to make a saturated solution. (Supersaturated)
3. Now take the content of this test tube into another clean and dry test tube and add few drops of distilled water and allow it to cool for some time.
4. A precipitate appears at the bottom of the test tube.
5. Carefully, transfer the precipitate on a glass slide and observe under microscope.
INTERPRETATION:
• When rhombic shaped cholesterol crystals are examined under the microscope and its shows a notch appearance.

OSPE QUESTIONS:
1. Perform cholesterol crystal test and gives inferences and principle of this test?
2. Draw the diagram of cholesterol and gives its clinical importance
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